Ding L, Zhao C, Qu J, Li Y, Sugiarto G, Yu H, *Wang J, *Chen X. A Photobacterium a2–6-sialyltransferase mutant (Psp2,6ST) with an increased expression level and improved activities in sialylating Tn antigens. Carbohydr. Res. 2015, 408, 127-133. PMID: 25593075. DOI: 10.1016/j.carres.2014.12.007.

‡Huynh N, ‡Li Y, Yu H, Huang S, Lau K, *Chen X, *Fisher AJ. Crystal structure of sialyltransferase from Photobacterium damselae. FEBS Lett. ‡Co-first authors. 2014, 588, 4720-4729. PMID: 25451227. PMCID: PMC4268365. DOI: 10.1016/j.febslet.2014.11.003. ‡Co-first authors.

‡Sugiarto G, ‡Lau K, Qu J, Li Y, Lim S, Mu S, Ames JB, *Fisher AJ, *Chen X. A sialyltransferase mutant with decreased donor hydrolysis and reduced sialidase activities for directly sialylating Lewisx. ACS Chem. Biol. 2012, 7, 1232-1240. PMID: 22583967. PMCID: PMC3521065. DOI: 10.1021/cb300125k ‡Co-first authors.

Thon V, Li Y, Yu H, Lau K, *Chen X. PmST3 from Pasteurella multocida encoded by Pm1174 gene is a monofunctional a2–3- sialyltransferase. Appl. Microbiol. Biotechnol. 2012, 94, 977-985. PMID: 22075637. DOI: 10.1007/s00253-011-3676-6.

Thon V, Lau K, Yu H, Tran BK, *Chen X. PmST2 – A novel Pasteurella multocida glycolipids alpha2–3-sialyltransferase. Glycobiology. 2011, 21, 1206-1216. PMID: 21515586. PMCID: PMC3150114. DOI: 10.1093/glycob/cwr054.

Sugiarto G, Lau K, Li Y, Khedri Z, Yu H, Le D-T, *Chen X. Decreasing the sialidase activity of multifunctional Pasteurella multocida alpha2–3-sialyltransferase 1 (PmST1) by site-directed mutagenesis. Mol. BioSyst. 2011, 7, 3021-3027. PMID: 21858283. DOI:10.1039/C1MB05182B.

Sugiarto G, Lau K, Yu H, Vuong S, Thon V, Li Y, Huang S, *Chen X. Cloning and characterization of a viral alpha2–3-sialyltransferase (vST3Gal-I) for the synthesis of sialyl Lewisx. Glycobiology. 2011, 21, 387-396. PMID: 20978012. PMCID: PMC3033747. DOI:10.1093/glycob/cwq172.

Ding L, Yu H, Lau K, Li Y, Muthana S, Wang J, *Chen X. Efficient chemoenzymatic synthesis of sialyl Tn antigen and derivatives. Chem. Commun. (Camb) 2011, 47, 8691-8693. PMID: 21725542. PMCID: PMC3201767. Web-themed issue on Glycochemistry & Glycobiology. DOI: 10.1039/c1cc12732b.

Cheng J, Yu H, Lau K, Huang S, Chokhawala HA, Li Y, Tiwari VK, *Chen X. Multifunctionality of Campylobacter jejuni sialyltransferase CstII: Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities. Glycobiology. 2008, 18, 686–697. PMID: 18509108. PMCID: PMC2588429. DOI:10.1093/glycob/cwn047.

Sun M, Li Y, Chokhawala HA, Henning R, *Chen X. N-terminal 112 amino acid residues are not required for the sialyltransferase activity of Photobacterium damsela a2,6-sialyltransferase. Biotechnol. Lett. 2008, 30, 671–676. PMID: 17989925. PMCID: PMC2598773. DOI: 10.1007/s10529-007-9588-y.

Ni L, Sun M, Yu H, Chokhawala H, *Chen X, *Fisher AJ. Cytidine 5’-monophosphate (CMP)-induced structural changes in a multifunctional sialyltransferase from Pasteurella multocida. Biochemistry. 2006, 45, 2139–2148. PMID: 16475803. DOI: 10.1021/bi0524013.

Ni L, Chokhawala HA, Cao H, Henning R, Ng L, Huang S, Yu H, *Chen X, *Fisher AJ. Crystal structures of a Pasteurella multocida sialyltransferase complexes with acceptor and donor analogs reveal substrate binding sites and catalytic mechanism. Biochemistry. 2007, 46, 6288–6298. PMID: 17487984. DOI: 10.1021/bi700346w.

Li Y, Sun M, Huang S, Yu H, Chokhawala HA, Thon V, *Chen X. The Hd0053 gene of Haemophilus ducreyi encodes an alpha2,3-sialyltransferase. Biochem. Biophys. Res. Commun. 2007, 361, 555–560. PMID: 17662691. PMCID: PMC2084346. DOI:10.1016/j.bbrc.2007.07.063.

Yu H, Huang S, Chokhawala H, Sun M, Zheng H, *Chen X. Highly efficient chemoenzymatic synthesis of naturally occurring and non-natural a-2,6-linked sialosides: a P. damsela a-2,6-sialyltransferase with extremely flexible donor substrate specificity. Angew. Chem. Int. Ed. 2006, 45, 3938–3944. PMID: 16721893. PMCID: PMC2728590. DOI: 10.1002/anie.200600572.

Yu H, Chokhawala H, Karpel R, Yu H, Wu B, Zhang J, Zhang Y, Jia Q, *Chen X. A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries. J. Am. Chem. Soc. 2005, 127, 17618–17619. PMID: 16351087. DOI: 10.1021/ja0561690.

 

Reviews

McArthur JB, *Chen X. Glycosyltransferase engineering for carbohydrate synthesis. Biochem. Soc. Trans. 2016, 44, 129-142. PMID: 26862198. DOI:10.1042/BST20150200.

Li Y, *Chen X. Sialic acid metabolism and sialyltransferases, natural functions and applications. Invited Mini-Review. Appl. Microbiol. Biotechnol. 2012, 94, 887-905. PMID: 22526796. PMCID: PMC3534974. DOI: 10.1007/s00253-012-4040-1.